Differential DHS sites with decreased chromatin accessibility relative to rhesus macaque occur more commonly near transcription start sites (TSS), while those with increased chromatin accessibility occur more commonly distal to TSS. We found that nondifferential DHS sites are enriched for nucleotide conservation. The joint model provides a principled approach to distinguishing single from multiple chromatin accessibility changes among species. We identified 89,744 DHS sites, of which 41% are identified as differential between species using the joint model compared with 33% using the conventional pairwise approach. To illustrate, we applied our joint approach to DHS sites in fibroblast cells from five primates (human, chimpanzee, gorilla, orangutan, and rhesus macaque). Our approach employs a single quantitative test which is more sensitive than existing pairwise methods. Our method overcomes several limitations inherent to conventional threshold-based pairwise comparisons that become increasingly apparent as the number of species analyzed rises. To address this important gap in knowledge, we developed a new method to identify DNase I Hypersensitive (DHS) sites with differential chromatin accessibility between species using a joint modeling approach. Neither RefWorks or EndNote have figured out how to write your papers for you, but both are excellent tools for managing and formatting citations.Changes in transcriptional regulation are thought to be a major contributor to the evolution of phenotypic traits, but the contribution of changes in chromatin accessibility to the evolution of gene expression remains almost entirely unknown. Web-based, so will work on any computer w/ internet Access through any computer(s) in which you’ve installed EndNoteĬollaborative projects, term papers, courseworkĬomplex research projects, dissertations, lengthy tomes Web-based (Any computer w/ Internet access) Lucky for you, Duke has a subscription to both, so the choice is yours! In the competitive world of Ultimate Citing, two kingpins rule the ring…RefWorks and EndNote, the academic world’s leading bibliographic management tools.
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